In the semi-final match held on April 17, 2025, at Aimane Stadium, Pranburi District, Prachuap Khiri Khan Province, the defending champion, Suan Sunandha Rajabhat University, faced off against the former runner-up, Kasem Bundit University, in the first match of the round.

The game ended in dramatic fashion as Suan Sunandha Rajabhat University scored the winning goal in the final 31 seconds of the match, sealing a 2-1 victory and securing a place in the final to defend their championship title for a second time.

Meanwhile, Kasem Bundit University, who missed the chance to reach the final for the first time in five editions of the tournament, will compete in the third-place playoff.